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skeletal muscle basal medium  (PromoCell)


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    Structured Review

    PromoCell skeletal muscle basal medium
    Skeletal Muscle Basal Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/skeletal+muscle+basal+medium/Skeletal+Muscle+Cell+Basal+Medium%2C+phenol+red-free/bio_rxiv__64898__2026__04__27__721147-34-15-14
    Average 94 stars, based on 11 article reviews
    skeletal muscle basal medium - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: Effect of human myoblasts on tenogenic progression in 2D and 3D culture models.
    Article Snippet: In brief, muscle tissues were cut into small pieces (<0.1 cm3) after removing all visible non- muscle tissue and digested for 1 h with Skeletal Muscle Basal Medium (PromoCell, Heidelberg, Germany; C- 23260) containing 0.2% type B collagenase (Roche Sigma Aldrich, Darmstadt, Germany; 11,088,815,001) and 0.2% dispase 170 II (Roche Sigma Aldrich; D4693- 1G) at 37°C, in 5% CO2.

    Recombinant:

    Article Title: MicroAge Mission: Experimental Design, Hardware Development and Operational Considerations for a Bespoke Culture System to Support Tissue-Engineered Skeletal Muscle Constructs
    Article Snippet: Unless stated otherwise, all other chemicals used in this study were obtained from Sigma Aldrich, Dorset, UK. .. Human immortalised myoblasts (AB1167_CTL) were routinely maintained in a complete growth medium consisting of PromoCell skeletal muscle basal medium (phenol red-free) supplemented with 20 % (v/v) FBS, 50 μg/mL bovine fetuin, 10 ng/mL epidermal growth factor, 1 ng/mL basic fibroblast growth factor, 10 μg/mL recombinant human insulin, 0.4 μg/mL dexamethasone, 10 μg/ mL gentamicin and 4 mM L-glutamine , . ..

    Article Title: MicroAge mission: experimental design and hardware for a bespoke culture system supporting tissue-engineered skeletal muscle.
    Article Snippet: Unless stated otherwise, all other chemicals used in this study were obtained from Sigma Aldrich, Dorset, UK. .. Human immortalised myoblasts (AB1167_CTL) were routinely maintained in a complete growth medium consisting of PromoCell skeletal muscle basal medium (phenol red-free) supplemented with 20 % (v/v) FBS, 50 g/mL bovine fetuin, 10 ng/mL epidermal growth factor, 1 ng/mL basic fibroblast growth factor, 10 g/mL recombinant human insulin, 0.4 μg/mL dexamethasone, 10 g/ mL gentamicin and 4 mM Lglutamine 19,20. ..

    Article Title: MicroAge mission: experimental design and hardware for a bespoke culture system supporting tissue-engineered skeletal muscle
    Article Snippet: Unless stated otherwise, all other chemicals used in this study were obtained from Sigma Aldrich, Dorset, UK. .. Human immortalised myoblasts (AB1167_CTL) were routinely maintained in a complete growth medium consisting of PromoCell skeletal muscle basal medium (phenol red-free) supplemented with 20% (v/v) FBS, 50 μg/mL bovine fetuin, 10 ng/mL epidermal growth factor, 1 ng/mL basic fibroblast growth factor, 10 μg/mL recombinant human insulin, 0.4 μg/mL dexamethasone, 10 μg/ mL gentamicin and 2 mM L-glutamine , . ..

    Article Title: MicroAge Mission: Effects of Microgravity and Heat Shock Protein 10 Overexpression on the Proteome of Human Tissue-Engineered Muscle Constructs - Implications for Skeletal Muscle Ageing
    Article Snippet: Unless stated otherwise, all other chemicals used in this study were obtained from Sigma Aldrich, (Dorset, UK). .. Human immortalised myoblasts (AB1167) were routinely maintained in a complete growth medium consisting of PromoCell skeletal muscle basal medium (phenol red-free) supplemented with 20 % (v/v) FBS, 50 μg/mL bovine fetuin, 10 ng/mL epidermal growth factor 1 ng/mL basic fibroblast growth factor, 10 μg/mL recombinant human insulin, 0.4 μg/mL dexamethasone, 10 μg/ mL gentamicin and 2 mM L-glutamine. ..

    Cell Culture:

    Article Title: Myo-MOVES: a custom electrical stimulation system for functional studies of 3D bioengineered muscle
    Article Snippet: .. Cells were cultured in growth medium consisting of skeletal muscle basal medium (C-23060, PromoCell GmbH), skeletal muscle supplemental mix (C-39365, PromoCell GmbH), 10% fetal bovine serum (FBS) (10270-106, GibcoTM) and 100 U mL −1 penicillin and 100 μg mL −1 streptomycin (15140-122, GibcoTM). ..

    Article Title: Patient-derived 3D engineered human muscle model recapitulates CLCN1 mis-splicing and myotonia in myotonic dystrophy type 1
    Article Snippet: .. Cells were cultured at 37 °C in 5% CO 2 and expanded in growth medium (GM) consisting of skeletal muscle basal medium supplemented with skeletal muscle supplement mix (PromoCell), 10% (v/v) fetal bovine serum (Gibco), and 100 U ml -1 penicillin with 100 μg ml -1 streptomycin (Gibco). .. Differentiation medium (DM) was based on Dulbecco’s Modified Eagle Medium, high glucose, GlutaMAXTM Supplement (Gibco), containing 1% (v/v) KnockOutTM Serum replacement (Gibco), 1% (v/v) Insulin-Transferrin-Selenium-Ethanolamine (Gibco), 1% (v/v) Penicillin-Streptomycin-Glutamine (Gibco), 100 ng ml -1 human agrin (Sigma-Aldrich), 50 ng ml -1 human Insulin-like Growth Factor-1 (IGF-1, Sigma-Aldrich), and 1 mg ml -1 6-aminocaproic acid (Sigma-Aldrich).

    Article Title: A comparison of human skeletal muscle cell maturation in 2D versus 3D culture: A quantitative proteomic study
    Article Snippet: .. Proliferating myoblasts were split using standard methods at 70% confluency and cultured in complete growth medium consisting of Skeletal Muscle Basal Medium supplemented with 50 μg/mL bovine fetuin, 10 ng/mL human epidermal growth factor, 1 ng/mL human basic fibroblast growth factor, 10 μg/mL human insulin, 0.4 μg/mL dexamethasone (all contained in C‐23060; PromoCell, Heidelberg, Germany), 20% (v/v) foetal bovine serum (FBS; 16140071; Gibco, Waltham, MA, USA), 2 mM L‐glutamine (59202C; Gibco, Waltham, MA, USA) and 10 μg/mL gentamicin (G1397; Sigma‐Aldrich, St. Louis, MO, USA). ..



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